An overview of the expression profile of all genes in shoot from rice seedlings treated with brassinolide (BL). Seeds of japonica rice cultivar Nipponbare were germinated and grown hydroponically in a growth chamber at 28ºC under continuous light. Seven-day old seedlings were transferred in culture solution with 1 μM BL and in culture solution without BL to serve as control (mock treatment). Shoot samples were collected at 0 min (pretreatment), and after 1 h, 3 h, 6 h and 12 h incubation (Details of methods
). The RNA isolated from a total of 10 samples in two replicates were labeled with Cy3 (mock treatment) and Cy5 (hormone treatment), and used for hybridization using the Agilent two-color microarray analysis system (Sample list
). The time-course expression profile for each gene is shown as raw data representing Cy3 and Cy5 signal intensity and the log-ratio of signal intensity (Log2
Cy5/Cy3). Search options include keyword search for individual RAP-DB locus ID, MSU Osa1 Rice Loci, and accession number, or chromosome search to get a list of all genes for each chromosome.